Journal: The Journal of experimental medicine
Article Title: Lung tissue-resident memory T cells optimize protection by IL-10 regulation of innate immunity
doi: 10.1084/jem.20242307
Figure Lengend Snippet: (A) Quantification of total CD4 + and CD8 + T cells in the lungs of mice treated with anti-Thy1.2 depleting Abs (orange) or IgG control Abs (gray). Statistical significance was determined by the student’s t-test. Boxes indicate interquartile range with a line drawn at the mean; whiskers indicate the minimum and maximum values. (B) T RM content in the lungs of T cell-depleted or control mice in (A) shown in representative flow cytometry plots (left) and compiled results (right) of CD45 expression from i.v. Ab administration (CD45(IV), left) and CD69 expression (right) by CD4 + (top), and CD8 + (bottom) T cells. (C) Lung macrophage accumulation after influenza infection in T RM -depleted (blue) and control (green) mice undergoing secondary infection at 4dpi compared to uninfected counterparts shown in representative flow cytometry plots (left) and compiled results (right) of F4/80 + macrophages in the lungs. Statistical significance for (A-C) was determined by the student’s t-test. (D-E) Pearson correlation analyses between T RM and CD4 + and CD8 + T RM subsets with (D) macrophages and with (E) Arg1 + macrophages. Data are representative of two independent experiments, with n=7–10 mice per group. ****, p ≤ 0.0001; ***; p ≤ 0.001; **, p ≤ 0.01; *, p ≤ 0.05.
Article Snippet: To deplete T RM from mice, WT mice were infected with X31 influenza virus as above and 3 weeks post infection (wkpi), the resultant memory mice were administered 10μg/g of Thy1.2-depleting antibody (BioXcell; BE0066) by intraperitoneal (IP) injection four times over the course of one week.
Techniques: Control, Flow Cytometry, Expressing, Infection
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